phosphorylated paris  (Cell Signaling Technology Inc)

Journal: BMB Reports

Article Title: Gefitinib induces anoikis in cervical cancer cells

doi: 10.5483/BMBRep.2023-0225

Figure Lengend Snippet: Gefitinib-induced reorganization of actin cytoskeleton and alteration of cell-ECM adhesion molecules. (A) HeLa cells were treated with or without 40 μM gefitinib for 24 h and analyzed using confocal microscopy. Actin cytoskeleton was labeled with fluorescent phalloidin (red), and nuclei were stained with DAPI (blue). Selected areas of merged images (scale bars, 40 μm) are shown at high magnification (right panels, scale bars; 10 μm). (B) Actin polymerization was assayed by measuring the fluorescence intensity of phalloidin bound to polymerized actin. The RFI is the ratio of the fluorescence intensity of the gefitinib-treated cells to that of the untreated control cells. (C) HeLa cells were treated with the indicated concentrations of gefitinib for 24 h (left) or with 40 μM gefitinib for the indicated durations (right). c-Abl and phosphorylated c-Abl (Tyr245), (D) cell-ECM adhesion molecules, and (E) E-cadherin were analyzed using western blot. P values were determined by Student’s t -test (***P < 0.001).

Article Snippet: Antibodies against PARP, caspases, cell-ECM adhesion molecules, c-Abl, phosphorylated c-Abl, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Confocal Microscopy, Labeling, Staining, Fluorescence, Control, Western Blot